Daisy took to her Instagram page to share the news of opting out of the film, which stars Ram Charan and Jr. NTR.
"RRR, unfortunately, due to family circumstances I am unable to participate in filming for this wonderful film. The script is truly brilliant and it's such a great character. I hope whomever they cast, receives as warm a welcome as I have and I wish the production all the best," Daisy posted on her Instagram page.
In the film, Daisy was supposed to be paired with Jr. NTR.
A tweet from the official account of RRR read: "Due to unavoidable circumstances, Daisy Edgar Jones is no longer part of our film. We hope she has a brilliant future."
Last month, speaking to reporters, Rajamouli said that RRR, which is being made on a lavish scale, will be a fictional story based on two real-life heroes and freedom fighters - Alluri Seetharama Raju and Komaram Bheem.
While Jr. NTR, otherwise known as Tarak, will be seen as Komaram Bheem, Ram Charan will be playing Alluri Seetharama Raju.
RRR also stars Ajay Devgn, Alia Bhatt and Samuthirakani in key roles.
The film will hit the screens worldwide on July 30, 2020 in 10 Indian languages.
Images: Daisy on Instagram