Just when the duo seemed to be cruising along comfortably, Pattinson provided the breakthrough when he came back for his second spell by accounting for Pujara.
Pujara fell to an off-cutter in the 29th over after striking six fours in his 76-ball stay at the crease.
With three days left in the game, India will look to come close to the Australian total and then push to seize the initiative.
Earlier, both the openers were out bowled. Vijay was the first to go after a 15-ball stay, which included two fours, done in by Pattinson’s pace.
In Image: Sachin plays a stroke